Foods and Feeding
Vinegar Eels
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Vinegar Eel or Turbatrix aceti  are a small nematode worm that is both  harmless and non parasitic they feed on a culture medium known as mother  of vinegar an ingredient used in the making of vinegar. Vinegar today  is pasteurised in much the same way as milk to rid the liquid of these  small worms.
 We as aquarists/fish breeders can utilise these small nematodes to feed very small fry.
 
 Initally you will need is a small quantity of Vinegar Eel starter culture available from various sources. (Thanks Donald)
 
 The everyday items needed to culture Turbatrix aceti are:
 Jam Jars
 Cotton handkerchiefs and elastic bands
 Red or White Wine or Cider Vinegar
 Apple Slices
 
 
 All you need to start your Vinegar eel cultures
 
 
 Fill the Jam Jar half full of your preferred Wine/Cider Vinegar.
 Some aquarists like to dilute the vinegar with a little water but I like to use mine neat.
 
 
 Next take your starter culture and pour some into the Wine/Cider Vinegar in the jam jar.
 
 
 Add a slice of apple. This will sink over the comming days.
 
 
 Finally cap with a slice of cotton handkerchief and secure with an elastic band.
 This lets the culture breathe and stops it getting infested with flies.
 
 These cultures are best kept warm [room temperature] in the dark and covered as mentioned above.
 
 Harvesting
 
 Method 1:
 Pour the medium siphoned off your culture through coffee filter paper.  Keep the vinegar; return this to your culture. Now rinse the eels  trapped in your filter by running some fresh water through this. Finally  invert into a glass of clean water. Leave them in the water to purge  them before feeding.
 
 Method 2:
 Siphon off some of your culture from near the top into a long necked  bottle [i.e. a wine bottle]. Fill the bottle until the vinegar just  starts to enter the neck of the bottle. Then take a small amount of  filter wool to form a plug. Push this down until it sits on the vinegar,  leaving most off the neck empty. Fill it up with fresh water. The eels  will crawl through the filter floss into the fresh water. They can then  be harvested with an eye-dropper or turkey baster, and fed to your fry.
 
 Another little livefood for small fish fry that can be cultured by the home aquarist for you to try
 
 ATB
 C
 
 Further interesting reading
 www.waynesthisandthat.com/eels.htm
Culturing Whiteworm
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Enchytraeus albidus (Whiteworm)
 
 Most aquarists, if not already done so, will one day want to culture  some live-foods to feed to their beloved fish. Whiteworm are just one of  a myriad of nematodes that can be cultured quite easily at home.
 A small amount of time and devotion and attention to detail will reward you with many sub-cultures for your fish larder.
 
 
 1) Fully soak your bread, I like to use Hovis Granary, I find it's the best for my purposes.
 
 
 2) Lay your saturated bread onto your worm culture.
 
 
 3) Now let them tuck in.
 
 
 4) Jeez..at this rate I'm gonna need another loaf 
 
 
 Temp
 The optimum temperature for reproduction is 15° - 21° C. Below 9°C the worms will stop breeding and above 27°C they will die.
 
 Breeding
 For breeding you will need the following:
 • A Container; e.g. an ice-cream tub or similar with holes punched in the lid. Pinholes will suffice so as not to attract fly infestation.
 • Medium; Potting Compost; I make my own with a little garden  lime added, as worms do not like acid soil. Aquatic soil is also a good  medium.
 • Food; To begin with I use granary bread cut into 1” squares and  soaked in milk. when the Culture matures I then use full slices of  Brown Granary Bread fully soaked in RO water, this method has presented  me with my best results in terms of yield thus far.
 Place your compost in your container to about three inches deep and  gently firm. Spray this until it is nice and damp, but not soaking wet.  Empty your starter into a depression in the centre of the container.  Gently level this and add a square of bread to begin with. Cover this  with a piece of glass or slate (the later works best). Cover the box as  white worm’s don’t like light.
 The worms will begin to slowly disperse into your culture box. To begin  with the worms will eat very little and you may find that the food will  grow a white hairy fungus. If this is the case carefully remove the  uneaten food, taking care not to remove the worms that are attached to  the base of the rotting food. Replace with fresh food. Check the culture  every day to ensure that they have enough food. You will find that as  the worms multiply, the food will be consumed more quickly.
 You can increase the number of cultures by adding some worm filled media  from your new culture once it is established (as you did to start your  first culture), or upsize to a larger container.
 Your culture should be ready to harvest in about 8 - 12 weeks.
 
 Harvesting
 A common fault is that people attempt to harvest too early. It usually  takes at least 8 weeks for the worms to be collected in numbers large  enough to both feed fish and sustain the culture.
 To collect the white worm place them in a plastic tub containing a small  amount of water for around 5 minutes (this is long enough for the worms  to purge themselves of any compost), by this time they will have  collected together in small balls and are easy to collect and feed to  fish.
 
 I hope this small article of another easy to culture livefood encourages some folks to give it a go.
 
 Regards
 C
Culturing Mexican Gammarus (Hyalella aztec)
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by
Puddlefish
 
 
 Introduction:
 I first came across this little species of shrimp whilst discussing  livefood sources with a fellow longstanding killifish enthusiast. He  imparted to me that this species would remain (even when adult) small  enough for average sized killi species to consume. They would also  proliferate at such a speed that a 24x12x12 tank set aside for such a  purpose would feed a whole fish-house weekly. What more could I want. He  offered to send me a culture and from that contact, this article was  spawned. I hope you enjoy this new editoral and be encouraged to seek  and find more natural and obscure food resources for your fishes.
 Hyalella aztec as the second part of the latin name suggests  hails originally from South America (Mexico). They are maintained in  many laboratories around the world as they are extensively used in  toxicological studies when assessing the environmental health of rivers,  streams and other watercourses.
 
 
 Mexican Gammarus shrimp x200
 
 Life Cycle:
 This little amphipod will mate several times per year. If you observe  the courtship closely you will notice that the male will carry the  female on his back, for about a week, whilst swimming, during the  copulation process. Females will normally produce around 40-50  fertilised eggs in her brood pouch which show up as being orange in  colour and are conspicuous through her semi-transparent body.  The tiny  shrimp hatch within the egg pouch of the female and emerge as  microscopic, fully developed young.
 They will then develop an exo-skeleton and will shed this (moult)  several times as they grow. The shrimp have no natural defences against  predators so they will hide in any dark areas of the tank. They will  however become more active under low light conditions or after dark. 
 
 Apperance:
 Hyalella aztec, as with many other species of Gammarus Shrimp have a  body which is laterally compressed, they are thicker in their length  rather than width. The body is divided into eleven segments, one for  each set of appendages. There are two long antennas on the head ,  followed by two pairs of grasping legs, half way down their body they  have  five pairs of legs for walking, these are followed by another   pair of hind legs which assist the shrimp when feeding, curled head to  tail.
 The tail section has two small protrusions.
 
 Size:
 This little species is fairly small with adults perhaps reaching only  10-15 mm  in length. This is perfect for feeding to tropical fish  species of average size. (Males are larger than females)
 
 Colour:
 This depends entirely on their diet within their habitat. They are  semi-transparent and their entire digestive tract is visible through the  walls of their shell. They will scavenge on all manner of materials,  but most of the time they will adopt plant material as their primary  food source, of which algae will have an effect on their colouration,  showing up in the shrimp as various shades of green.
 Environmental surroundings can also effect the colouration. If subjected  to a habitat with lots of silt and mud as a substrate then the shrimp  will naturally appear to be brownish and blend into the surroundings  which help to camouflage them and protect them from becoming prey. You  will however always be able to spot the pregnant females by the colour  of her eggs being carried within her brood pouch.
 
 Movement:
 When you have a population of these going, you will notice that they  will swim for around 2 to 6 seconds, and then they will take a rest and  breathe. If they are not holding onto something within the tank they  will sink toward the bottom in a curled position after a few seconds  they will uncurl and swim another short distance. They need to have  constant circulation of water over their gills, to achieve this their  legs are constantly in motion.
 
 
 Male and Female coupled
 
 
 Habitat:
 Gammarus Shrimp are primarily scavengers they mostly feed on plant and  animal material that have settled to the bottom. Sometimes they will  adopt a predatory role and attack other injured or stressed aquatic  life. For the most part they tend to hide among plant matter and under  rocks or debris when not travelling or mating. Shrimp need calcium to  build their shells or exo-skeleton.
 So an environment which is non acidic and high in dissolved salts and calcium suit this little critter best.
 
 Home Aquaculture:
 When first acquiring my initial starting culture of this shrimp, I asked  many questions regarding their aquaculture and husbandry, all of which  were met by the same reply. 
 
 That said I just knew these little buddies were going to be a cinch.  Looking at the evidence above I would have said that this species were  nearly impossible to wipe out and you know that's not too far removed  from the truth.
 
 I initially set the shrimp up in a 3ltr ice cream tub. Rainwater  buffered with a little Bicarb of Soda and some crushed coral sand (in a  small tub). Added to this set-up a large Juwel brand coarse filter sponge was added. 
 
 
 It needs to be the course type as the shrimp utilise this for habitation  purposes much the same as being under stones in the stream or river.  Finally a few dead leaves were added. Being habitants of moving  waterways suggested that they like a lot of oxygenation in their water  so I added an airline which provided plenty of surface agitation to  their new home, all be it temporary. I feed the little beggars anything  that's lying around but predominately they get Tetra Pond Pellets. (must  try trout pellets from the local angling shop). They grasp on to these  pellets and feed, 100's at a time, its so comical to watch.
 In a tub that size I would feed 15-20 pellets a day and water-change every other day.
 The population explosion of this species has to be seen to be believed. I  have only had this new form of livefood a short while now, and its  truly amazing how these things reproduce so quickly. 
 I can see how it is possible to feed a whole fish-house on these and not deplete you initial stock.
 Harvesting the shrimp is very easy too, all you need is a small  container with a lid. Punch some holes in the lid and load the container  with some delicacy (pond sticks),replace the lid with a length of  string attached (so as you can retrieve the pot from the culture  aquarium) After a while draw up the pot and it should be filled with  Gammarus ready to be fed to your charges.
 
 Summary:
 If you are looking for a small sustainable form of livefood to be able  to feed your fish on a regular basis, then look no further than this  little species. Simplicity in itself to set-up and maintain. The fish  seem to love these critters and take them with gusto every time they are  offered.
 Note!
 One downside is that these critters should only be fed to breeding  aquaria and stark aquariums devoid of (or folks not worrying about their  greenery) plants. Due to the nature of things these shrimps will strip  plant matter from a tank very good for hair algae but will relish  aquarium plants as desert when that is used up. Sharing a tank with  Cherry or other small shrimp species is also a bad idea due to them  being out competed for food.
 Those small points aside and looking at things from a breeders  perspective, then this shrimp species ticks a lot of boxes when trying  to keep a fully stocked larder, especially over the winter months when  livefood becomes scarce.
 I hope some of the patrons of this site will give them a go.
 All imagery by the Author except Juwel Coarse Sponge pic
 Regards
 C
A Shrimp from the Orient
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Culturing Branchinella thailandensis
 A Shrimp from the Orient
 by
 Puddlefish
 
 
 This lengthy editorial was constructed by myself due to the sheer lack  of non technical reference material available to the hobbyist regarding  this interesting subject matter. I've scoured the internet for hours on  end for relevant data that I could use and I only managed to find  somewhat sketchy articles of which only just brushed on the topic. So as  an endeavour to try and help other aquarists and folks interested in  home aqua-culturing such creatures I have managed to put together  something based solely on my personal experience. I hope you enjoy what I  have managed to unearth.
 
 
 This being a new venture for me and for anyone that knows me will impart  that I like to culture live foods at home if possible, in an effort to  give my fish a different and diverse dietary range to satisfy their  everyday bodily needs. Borne out of the simplistic idea that brine  shrimp need brine water, a salty mix within a specific gravity range to  thrive and grow and we are predominately using freshwater as a medium  for our fish which is an alien environment for Artemia to survive much  longer than an hour or so. Subsequently Artemia do not live very long in  freshwater tanks or fry tubs, leading to the fouling of the water which  could, if left, lead to disease with resultant fish and fry losses.  Thai Fairy Shrimp on the other hand would possibly fill this gap.  offering me a high protein, low fat food source that would stick around  longer in a freshwater environment giving fish and fry a longer time to  catch and consume this fresh food source before ending up as a useless  mush on the aquarium floor. 
 
 Lots of aquarists would like to give this type of food source a go, but  shy away from attempts to home culture this small Crustacea species  due  to the fear of being duped by unscrupulous Thai vendors sending  unreliable cysts through the lengthy postal delivery system. So this  little article is set out with a two fold agenda, firstly to catalogue  my attempts at culturing and sustaining the shrimp to subsequent  populations and secondly to dispel the fear of ordering cysts from the  Far East.
 
 I'm not a fool... so realistically I would not be able to produce shrimp  cysts in such numbers as to be in a position to distribute large  quantities around the country. (I don't have a lake in my back yard)  what I am aiming for here is to produce enough shrimp and cysts with a  little effort to be able to have a self sustainable food source for both  fish and fry feeding. The initial program will be primarily to grow the  shrimp to adulthood and collect the cysts, this would be the number one  priority. To forget about feeding anything to the fish initially until I  have enough cysts to sustain future generations.
 An adult of 35mm would be of no use to myself as I only keep and breed  small Killifish species so for food purposes I would be only using  perhaps the 1st, 2nd and 3rd instars.
 
 
 What are Fairy Shrimp?
 There are many species of fairy shrimp catalogued geographically around  our planet, some small, some huge, but for the subject of this  particular article I will be concentrating on a relatively small species  from Thailand.Branchinella thailandensis. 
 According to published literature Branchinella thailandensis have been  found in the northeastern part of Thailand since 2002. This species can  potentially serve as live feed for fish and crustaceans in aquaculture  because of its high nutritional value; 64.94% protein, 5.07% fat,  17.96%. carbohydrate, 8.49% ash, providing an alternative to artemia  (56.4% protein, 11% fat, 12.1%. carbohydrate and 17.4% ash) 
 (Figures given suggest that they are of a much higher nutritional value  than our other most common freshwater food resource, Daphnia).
 
 
 The shrimp have adapted to living in shallow vernal pools, prone to  drying out on occasion as the seasons predict. The female lays hard  cased eggs (similar to Artemia cysts) that will lie dormant in the dry  substrate until the pool floods once again. From cyst to adult they go  through several instars. Culminating in mating and subsequent egg  laying. The lifecycle of the fairy shrimp takes roughly about 6-8 weeks  depending on temperature and food resources. Expect the adults reach a  size of between 25 to 35mm in this short time-frame. Fairy Shrimp are  filter feeders and can comsume wide range of food within the water  column such as bacteria, protozoa, algae, rotifer and organic matter. As  normal with this family of crustacean they are very sensitive to  adverse changes in water conditions and chemicals.  
 
 Acquisition of Cysts;
 
 
 Although the vendor states in his literature that he cannot be  responsible for hatching rates etc, I figure I should have enough cysts  and hopefully subsequent shrimp to get me to the next stage. Which  ultimately would be to raise as many shrimp as possible to adulthood and  collect the eggs they produce. 
 
 
 Aquaculturing;
 As with all my articles, the findings therein this particular study is  purely gathered from my own experiences on the subject matter. Many  aquarists may find different methods in order to gain equal or higher  success rates or yields. I call upon those people to make comments and  offer positive suggestions surrounding this material so as others may  share and educate themselves by reading these editorials.
 
 
 
 The Container;
 The first containers, like everything else are just standard jars and  tubs rather than specialist dedicated vessels specifically designed for  the job in hand. Initially I set up the cultures almost identical to my  internal  culturing of Daphnia pulex, in fact everything is essentially  the same or similar. Later as the shrimp grow on towards adulthood I  will move them into a proper all glass aquarium with a view to  collecting the eggs.
 
 
 When first setting up all of the vessels they have to be sterilised with  a very mild bleach solution after which they were thoroughly rinsed  with fresh water and meticulousness dried out. If you are attempting to  culture these crustaceans sometime in the future don't be tempted to use  other chemical washing-up liquids to clean your containers as the  residue they leave behind will kill the shrimp.
 
 The Water;
 The water I use for the shrimp is nothing fancy, just rainwater in a  clean sterile container with the pH adjusted to 7.6. by means of  Bicarbonate of Soda. The temperature is kept at around 22'C. Added to  the set-up is a small tub of Aragonite sand to help keep the pH up.
 
 The Cysts;
 You can differentiate Branchinella thailandensis cysts from other fairy  shrimp in the region just by observing the shape of their eggs casings.  Thai fairy shrimp cysts are spherical with many irregular six sided  indentations or dimples around its circumference. (see pic below)
 
 Branchinella thailandensis cysts under 200x magnification. (Artemia insert)
 
 You can see just by looking at the egg casings that they also differ from Artemia cysts (see insert) in that the casing is rough, heavily dimpled and not as smooth and uniform as the Brineshrimp cyst.
 
 
 Hatching;
 Now we come to the first step on our journey, the initial hatching of  the first batch of cysts. I have used a whole capsule of eggs for this  initial wetting. It is known from literature that all eggs must sink to  the bottom in order for them to hatch. Floating eggs wont hatch by all  accounts. You can use some coffee filter papers laid on top of the  floating cysts to get them to absorb more water and help them to  submerge. Any cysts that still manage to float you can re-dry (see later  in thread) and hatch at a later stage for more shrimp
 
 
 The cysts will immediately begin to absorb water, and will eventually  sink to the bottom of the jar. The baby shrimp will not hatch straight  away until they are convinced that the water body is permanent. This  will take about 24 to 48 hours, sometimes longer, depending on  conditions. After the allotted time the nauplii will hatch and begin  swimming with jerky movements as they propel themselves through the  water column. The do not need to be fed until the second day. They are  very small and a small (20x) lens or eye loupe will help you to see them  much better. They are "photo-tropic" so if you shine a beam of light  through the jar they will congregate near the light source and will be  more visible. Unlike brineshrimp vigorous agitation of the water is not  required to trigger fairy shrimp to hatch. 
 
 21/06/2010 The first batch  of eggs are prepared and readied for hatch as per above images. 24 hours  later, as expected, the first shrimp have hatched, lots of them, they  resemble artemia nauplii with  almost identical swimming patterns. My  little 200x microscope just isn't quite up to the task in these early  stages of life but should come into it's own in the days ahead.
 
 
 One observation....by comparison, the nauplii of Branchinella  thailandensis are almost transparent, opaque white in colour unlike  brineshrimp which are orange. I have administered a small feed (20ml) of  live algae to the culture jar, just enough to tinge the water green.
 
 Feeding;
 Fairy shrimp are filter feeders, They strain tiny particles out of the  water for food, in the wild this would mean mostly bacteria, algae, and  fungal spores. In captivity I feed them "Green Water" live algae which I  culture myself. (details of how to culture this green soup can be found  within my Daphnia pulex thread)
 
 
 You can determine how much food is enough by using this rule of thumb:  The water should be slightly hazy, but not cloudy. You should be able to  see through it. If it becomes cloudy, stop feeding for a day or two  until it clears up. If it becomes extremely clear, feed a little bit  more. Increase their food as they grow. At about 2 weeks, the shrimp  will need approximately 1 ml per day. When they are fully grown, they  will need up to 3 mls daily.
 
 Growth;
 Growth of fairy shrimp is pretty rapid, it has to be with such a short  lifespan. They will reach adulthood when they are about 12mm long. By  four weeks old, they should be close to 25mm long. On conferring with  shrimp farms in the far east, they seem to recommend a density of around  15 shrimp per litre of water once they reach adulthood. Here follows a  series of images cataloguing the growth of  Branchinella thailandensis.
 
  
 
 
  
 
 
 
 
 
 
 
 
 
 After the third week, (21 days) I again, transfer all the shrimp and  contents of their original container (3lb Ice Cream carton) into larger  quarters (an all glass tank 10x10x18). This allows them a much more  commodious space in which to grow out. Much more live algae is being  consumed than before. 200mls of the elixir is now being added which  gradually is being taken up by the shrimp, this murky soup clears after  48 hours to which another dose of food is administered. Large males are  starting to spar at this stage. Females are maturing and are carrying  white eggs awaiting fertilisation which will darken and form a hardshell  cyst after 24 hours, these cysts will subsequently be released to lay  dormant on the aquarium floor ready to be harvested in another month or  so. 
 
 
 
 Reproduction;
 Once the shrimp reach maturity they will concentrate exclusively on  breeding . The male's objective now is to fertilise a female to make new  viable cysts. The females will begin to produce eggs as soon as they  mature. The bright white eggs are stored in a transparent cigar shaped  pouch that is attached to their abdomen. This brood pouch makes the  female easy to identify. The male shrimp constantly swims around looking  for a suitable female. When he finds one, he will grab her with his  large antennae. The antennae look like walrus tusks attached to his  face. He will quickly fertilize her eggs and swim off to find another  suitable mate. You can tell when the eggs have been fertilized because  they will grow larger and turn a pale tan colour as they begin to  develop a cyst coating. Once the cysts are fully coated, the female will  push them out of her pouch, where they will sink to the bottom of the  container.
 
(ii) B. thailandensis cysts litter the substrate of the container
 
 The little embryos inside will grow and develop for about a day or two.  When they reach the point of about 4,000 cells they will shut down and  become dormant. They will not hatch until they are completely dried out,  chilled and re-wetted. One point to note here is, that from published  literature, which recommends that it is better to leave the cysts in the  parent aquarium for around two to four weeks after all shrimp have  expired which allows the resting shrimp embryo to fully develop before  entering its diapause (resting) stage. 
 
 The Next Generation;
 Afer 6-8 weeks the shrimp will start to die off due to their life cycle  having run its course, we should, by this time have many cysts strewn  all around the floor of their container. You cannot hatch these eggs  straight away. They have to be harvested, dried and refrigerated for a  week or so before starting the cycle all over again. 
 To harvest the cysts I use a clean meat/turkey baster and suck all the  gunk from the floor of the container, I then squirt this through coffee  filters. One full baster full of gunk laden water to each coffee filter  is ample.
 
(ii) Freshly collected substrate from the base of the Shrimp's aquarium
 
 I open out the coffee filter fully by carefully tearing down the seam.  The filter is then outspread on layers of paper hand towel to soak up  the excess water. This can now be left to dry out fully on top of the  aquarium cover glass.
 
(ii) Newly collected Shrimp eggs under 200x magnification
 
 Don't worry about harming the shrimp as they are safely encapsulated  inside their hard cased shell. Once fully dried you can place the  collected gunk into a small plastic bag and place in the fridge for  around a week (this seems to yield better results for subsequent  hatches). After this allotted time I can then start the process over  again only this time around as with subsequent hatches I can sacrifice  some of the shrimp as fish food. After a period of time the fish will  reap the rewards more and more as your stock of shrimp cysts increases.
 
 
 
 Observations;
 21/06/2010 - Initial setting up and egg immersion
 30/06/2010 - Not all  the shrimp seem to be developing at the same rate and are maturing at  different times              -  due to the probability that not all eggs  might have been laid/hatched at the same time.
 10/07/2010 - White eggs become apparent in brood pouches of 10mm females - Maturing males sparring.
 11/07/2010 - Eggs within above females darken in colour (hardshell cyst formation)
 13/07/2010 - It is noticeable that a great many eggs are strew amongst the debris on the base of the container
 21/07/2010 - I  sacrificed a medium sized shrimp today and placed it into a breeding  tank containing adult Rivulus punctatus. The shrimp was taken with  relish by a lucky female. I now know that the fish will eat the shrimp.
 
 Future Developments;
 This project was a scratch at the surface, a tentative approach to  shrimp culturing. For the future I will be using adapted upturned 15  litre water vessels, the type used on drinks dispensers within foyers of  office blocks. I'm also looking into the prospect of upping my algae  production by purchasing a couple of Aqua Medic Plankton Reactors (see  below) and adding CO2. This I feel is both a necessity and a natural  progression if I'm to feed my cultures of Daphnia pulex and Moina as  well as the shrimp.
 
 
 Conclusion;
 Seeing as I'm essentially only a hobbyist dabbling in culturing certain  terrestrial and aquatic live-foods this operation is diminutive as  shrimp aquaculture goes, it's adequate for my present needs. I do feel  however that I would get better results by scaling up to larger vessels  and dedicated filtration etc. something I'm in the process of accessing.
 I hope you have enjoyed this lengthy insight into the way I culture Thai  Fairy Shrimp. It's a fairly easy process if certain parameters are  followed. Admittedly it takes a little while to amass a decent quantity  of cysts to begin feeding to the fish, but whilst you wait for this to  happen you can enjoy observing the life-cycle of this truly amazing  little creature. 
 Thanks for viewing and I hope you have enjoyed reading this detailed editorial as much as I've enjoyed compiling it.
 
 
 Additional Reference Material
 The cyst hatching pattern of the  Thai Fairy Shrimp, Branchinella thailandensis Sanoamuang, Saengphan  & Murugan, 2002 (Anostraca)
 
 All photographs by the author (except Aqua Medic Reactors) please do not use without prior permission.
 Regards
 C
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
 
 UPDATE
 Just a little update on the harvesting of B. thailandensis.  It has struck me that the coffee filter method may be a little  haphazard as when the filter becomes soggy some eggs are lost through  the paper.
 What I would now recommend is to suck all the gunk from the base of the  aquarium into a small glass jar each time waiting for everything to  settle then extract as much of the water as possible (syringe and  airline attached). Once you have everything collected and most of the  excess water extracted, then, simply leave the jar in a warm place (top  of an aquarium light canopy or fish-house shelf) and allow the rest of  the moisture to evaporate naturally. Once totally dry, cap the jar and  place in the fridge for around a week. After which, simply take you jar  laden with  shrimp eggs from the fridge and place this into a larger  vessel filled with water and let the shrimp hatch naturally from there.
 Regards
 Colin
Decapsulating Brine Shrimp eggs
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[b]De-capsulating Brineshrimp Eggs
A step by step pictorial[/b]
The problem with hatching brineshrimp eggs using conventional methods means that once hatched you are left with literally millions of unwanted shell casings, these inadvertently get fed along with the  newly hatched shrimp to your fry and corals, they are in fact quite sharp and can kill fry. So what can we do to keep fry losses to a minimum whilst separating those unwanted egg casings?
We can dissolve the outer shell of the Brineshrimp cyst by a process known as De-Capsulation.  
If you examine a Brineshrimp cyst under a strong lens or microscope you will see that it has the look of a deflated ball, lots of little dimples and a large indentation.
Before we begin the de-capsulation process we will need to make the outer shell spherical. Before we can start the process the outer shell must be fully hydrated. We do this by hydrating the amount of eggs you wish to de-capsulate in a water solution. 
 
Here is a picture of Brineshrimp Cysts before actual Hydration, I have seperated a little group to illustrate the concave shape of most of the eggs before immersing in water.
This picture shows the dramatic difference in the shape of the cyst after they have been immersed in water for 90 mins
The items we will be using are:-
Household Bleach (non Fragranced)
White Vinegar
2x Small beakers with lids
Cotton Handkerchiefs or Brineshrimp sieve
Brineshrimp eggs/cysts
Airpump & Airline
Brineshrimp hatchery.
As I only de-capsulate the amount of eggs I need for any one feeding, I will show you my methods and you can scale these up or down as need be to suit your own situation.
Take a small plastic beaker (mine holds around 250ml) and fill this to about a third of its volume with normal tap water, add to this one level teaspoon of Brineshrimp Eggs and replace the cap on the beaker. Shake the mixture vigorously and keep shaking this solution periodically over the course of 90 minutes or so to fully hydrate the eggs.
Eggs Settled
Eggs in Suspension (keep shaking the jar periodically)
Once you’ve done that remove the cap and add household bleach until the container is around three quarters full. Replace the cap and shake once and let the eggs settle for a few moments then shake the jar to really mix the bleach and the eggs in the solution. 
Notice the reaction starting to take place (foaming at the top of the liquid)
Within a few minutes you should notice a reaction taking place, the colour of the cyst should change from brown to grey to white to orange depending on the strength of the chemicals and the thickness of the shell. 
Start of the reaction process
It's working
Almost finished!
When you notice that most of the cysts are orange in colour (90%) STOP! The process immediately by pouring the solution into a cotton hankie draped over another jar. Now, pour a small amount of white vinegar over de-capsulated eggs and once you’ve done this rinse the eggs under the tap to rid them of the entire bleach and vinegar residue.  
Rinse with White Vinegar and with plenty of tap water
Finished Product - The Amber Nectar.
Once you have your de-capsulated eggs you can hatch them in the normal way, only this time you will have none of the annoying egg casings to contend with.
Ready for hatching using the normal conventional method.
This may seem a awful lot of trouble to go to in order to feed your fry or corals. Believe me it is well worth the effort, as you will attain a visually higher hatch rate along with clean no mess feeding. 
Why don’t you try it some time, see for yourself.
Larger amounts of eggs can be used along with large amounts of chemical agents for aquarists who need higher volume hatches to feed their fry. De-capsulated Brineshrimp can also be stored in a heavy brine solution (which effectively dehydrates the membrane thus allowing the aquarist to store the eggs for up to a month in the fridge. Of course you dont even have to hatch the nauplii you can feed the unhatched shrimp in the membrane, some say its better nutritionally for the fry. I'll leave you to decide.....
I hope this article was of some help to those aquarists who are fed up with hatching Brineshrimp and the constant battle of trying to separate shrimp from shell.
Regards
C
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