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Retirement and forum shutdown (17 Jan 2022)

Hi,

John Howell who has managed the forum for years is getting on and wishes to retire from the role of managing it.
Over the years, he has managed the forum through good days and bad days and he has always been fair.
He has managed to bring his passion for fish keeping to the forum and keep it going for so long.

I wish to thank John for his hard work in keeping the forum going.

With John wishing to "retire" from the role of managing the forum and the forum receiving very little traffic, I think we must agree that forum has come to a natural conclusion and it's time to put it to rest.

I am proposing that the forum be made read-only from March 2022 onwards and that no new users or content be created. The website is still registered for several more years, so the content will still be accessible but no new topics or replies will be allowed.

If there is interest from the ITFS or other fish keeping clubs, we may redirect traffic to them or to a Facebook group but will not actively manage it.

I'd like to thank everyone over the years who helped with forum, posted a reply, started a new topic, ask a question and helped a newbie in fish keeping. And thank you to the sponsors who helped us along the away. Hopefully it made the hobby stronger.

I'd especially like to thank John Howell and Valerie Rousseau for all of their contributions, without them the forum would have never been has successful.

Thank you
Darragh Sherwin

formalin

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25 Mar 2010 19:45 #1 by platty252 (Darren Dalton)
What percentage of formalin is used to preserve fish organs?

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25 Mar 2010 19:49 #2 by Daragh_Owens (Daragh Owens)
I don't know, but I have a bottle of this stuff and if you can make sense of the instructions, it might help:

Formaldehyde 40% GPC

500ml - About Formaldehyde 40%:

Has many uses but a popular one is as a raw material for making Buffered Formalin 10% or Formal Saline 10% or straight Formalin 10% for fixation in histology.

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25 Mar 2010 19:55 #3 by derek (Derek Doyle)
Replied by derek (Derek Doyle) on topic Re:formalin
think its 10%. do you have to cut open the fish to preserve organs?

30 tanks specialise in african cichlids, angelfish and various catfish

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25 Mar 2010 19:59 #4 by JohnH (John)
Replied by JohnH (John) on topic Re:formalin
Many years ago, in another century, I used to use Formalin (you could still buy it then) for preserving minnows and Sprats in glass jars as baits for Fishing.
I remember I used to use a 10% solution but this only stopped them deteriorating for a few months as they were a bit 'high' when I opened them a year or so later.
John

Location:
N. Tipp

We're just two lost souls swimming in a fish bowl - year after year.


ITFS member.



It's a long way to Tipperary.

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25 Mar 2010 20:00 #5 by platty252 (Darren Dalton)
Daragh_Owens wrote:

10% for fixation in histology.[/b]


Dam. I was given 37% and was told i would have to dilute it but they didn't know by how much.

I will get back on the phone tomorrow to check the strength needed to preserve some fish organs to be sent to the lab in Galway.

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25 Mar 2010 20:04 #6 by JohnH (John)
Replied by JohnH (John) on topic Re:formalin
I think 37% is how it's sold (it's quite volatile at high percentages, I think) have you tried goggling it?
John

Location:
N. Tipp

We're just two lost souls swimming in a fish bowl - year after year.


ITFS member.



It's a long way to Tipperary.

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25 Mar 2010 20:06 #7 by platty252 (Darren Dalton)
derek wrote:

think its 10%. do you have to cut open the fish to preserve organs?


Yes they wont take a live fish.
I have to send (individually) liver, gills, spleen, heart and kidneys.
The spleen will be tricky since the fish are only about 2-2 1/2".

Daragh i might have to drop in tomorrow to get some 10% off you if thats ok.

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25 Mar 2010 20:07 #8 by platty252 (Darren Dalton)
JohnH wrote:

I think 37% is how it's sold (it's quite volatile at high percentages, I think) have you tried goggling it?
John


The formalin i got came from a lab. for fish.

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25 Mar 2010 20:11 #9 by JohnH (John)
Replied by JohnH (John) on topic Re:formalin
Have you considered Alcohol, I have a friend who is an entomologist and he uses it for all his specimens. I think you can get that from the Chemist's?
John

Location:
N. Tipp

We're just two lost souls swimming in a fish bowl - year after year.


ITFS member.



It's a long way to Tipperary.

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25 Mar 2010 20:23 #10 by JohnH (John)
Replied by JohnH (John) on topic Re:formalin
platty252 wrote:

JohnH wrote:

I think 37% is how it's sold (it's quite volatile at high percentages, I think) have you tried goggling it?
John


The formalin i got came from a lab. for fish.

Sorry to 'keep on', but I think you use 10% solution (with remainder water) of the 37% solution you have been provided...does this make sense?
John

Location:
N. Tipp

We're just two lost souls swimming in a fish bowl - year after year.


ITFS member.



It's a long way to Tipperary.

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25 Mar 2010 22:07 #11 by platty252 (Darren Dalton)
Not to sure John.
So that would be 3.7% formalin?

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25 Mar 2010 22:38 #12 by JohnH (John)
Replied by JohnH (John) on topic Re:formalin
That's my interpretation, but I've emailed my entomologist friend in Scotland and am awaiting his response.
John

Location:
N. Tipp

We're just two lost souls swimming in a fish bowl - year after year.


ITFS member.



It's a long way to Tipperary.

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26 Mar 2010 00:28 #13 by scubadim (scubadim)
Replied by scubadim (scubadim) on topic Re:formalin
Hi Darren,
if you use the 37% then 1 part of that solution with 2 parts of water should roughly be 10% formalin solution?!
(more like 12) .if that's what Is used in histology it would be sufficient to keep cells "intact"?How long?
I'm curious to know:huh:
Dimitri

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26 Mar 2010 12:22 - 26 Mar 2010 12:24 #14 by JohnH (John)
Replied by JohnH (John) on topic Re:formalin
A couple of replies:

a] from my Entomologist friend:

"Yes it should be 9 parts water to 1 part of the formalin (37%)"



b] from Ian Millichip:

"Hi John,

Time is against me to give a full reply unfortunately,

but if Formalin is used then there are quite a few precautions that need to be followed:

-the Formalin has to be buffered (possibly slightly on the acidic side, and may depend upon tissue);
-unbuffered formalin is converted to formic acid and that may decalcify specimens (you'll get a mush)
-Formalin is not safe and workers need to consider the irritant effect
-Care needs to be taken with the storage jars....glass with polypropylene lids (and ideally polyethylene lining). Certain other materials will react with the formalin.
- the specimen to perservative ratio is important and the ideal varies from specimen size to specimen size and type.

The specimen would need to be 'fixed' and 'preserved'.

Now, alcohol (95%) is a good preservative but maybe not a great fixative.
Many people use, and it may even be the recommended method for holding specimens during a field trip.
The Herpetological Society of Ireland recommends to people to preserve their animals in alcohol if they are sending them for analysis, dissection or future preservation treatment.
There is little need to buffer the alcohol (so maybe more convenient to use)

Now, most of the notions of preservation to perfection are driven by the needs of museums.....but I would be sure that for 'single person' usage the alcohol preservation may do.

As I have to dash, then here is a link www.adfg.state.ak.us/pubs/afrb/vol3_n2/shiev3n2.pdf

ian"

Location:
N. Tipp

We're just two lost souls swimming in a fish bowl - year after year.


ITFS member.



It's a long way to Tipperary.
Last edit: 26 Mar 2010 12:24 by JohnH (John). Reason: correction

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27 Mar 2010 01:32 #15 by platty252 (Darren Dalton)
Thanks for all the replies and info.

I got the formalin 10% buffered to neutral from aqua-vet Ireland.
They said they use this strength because when it is higher than 10% the samples become harder.
They also supplied the sample containers.

The samples will only be in the formalin for a coupe of days at most.
They just had to go from Dublin to Galway. All going to plan they should be in Galway now.

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09 Apr 2010 22:24 #16 by Sean (Fr. Jack)
Replied by Sean (Fr. Jack) on topic Re:formalin


Why not keep your "late" wet pets frozen:

That would be a ecumenical matter!!!
Attachments:

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09 Apr 2010 22:26 #17 by Sean (Fr. Jack)
Replied by Sean (Fr. Jack) on topic Re:formalin
Why not keep your "late" wet pets frozen:

That would be a ecumenical matter!!!

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10 Apr 2010 00:35 #18 by platty252 (Darren Dalton)
Hi Sean the formalin was not for preserving the fish for myself. It was to send tissue samples from Dublin to Galway.

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10 Apr 2010 01:47 #19 by tom3179 (Tomasz Roj)
Replied by tom3179 (Tomasz Roj) on topic Re:formalin
I used to use 10% for insects for preserve them, and later I did dried them out. (display cabinets for university use - I had butterflies from eggs to mature form :]). For fish 40% it's OK, but it is strong and organs can change colours etc. 10% is OK if you will use hermetic jars etc., without air acces. 10% i used to use to preserve fishing trophies many years ago.

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