Retirement and forum shutdown (17 Jan 2022)
Hi,
John Howell who has managed the forum for years is getting on and wishes to retire from the role of managing it.
Over the years, he has managed the forum through good days and bad days and he has always been fair.
He has managed to bring his passion for fish keeping to the forum and keep it going for so long.
I wish to thank John for his hard work in keeping the forum going.
With John wishing to "retire" from the role of managing the forum and the forum receiving very little traffic, I think we must agree that forum has come to a natural conclusion and it's time to put it to rest.
I am proposing that the forum be made read-only from March 2022 onwards and that no new users or content be created. The website is still registered for several more years, so the content will still be accessible but no new topics or replies will be allowed.
If there is interest from the ITFS or other fish keeping clubs, we may redirect traffic to them or to a Facebook group but will not actively manage it.
I'd like to thank everyone over the years who helped with forum, posted a reply, started a new topic, ask a question and helped a newbie in fish keeping. And thank you to the sponsors who helped us along the away. Hopefully it made the hobby stronger.
I'd especially like to thank John Howell and Valerie Rousseau for all of their contributions, without them the forum would have never been has successful.
Thank you
Darragh Sherwin
formalin
- platty252 (Darren Dalton)
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- Daragh_Owens (Daragh Owens)
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Formaldehyde 40% GPC
500ml - About Formaldehyde 40%:
Has many uses but a popular one is as a raw material for making Buffered Formalin 10% or Formal Saline 10% or straight Formalin 10% for fixation in histology.
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- derek (Derek Doyle)
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30 tanks specialise in african cichlids, angelfish and various catfish
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- JohnH (John)
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I remember I used to use a 10% solution but this only stopped them deteriorating for a few months as they were a bit 'high' when I opened them a year or so later.
John
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We're just two lost souls swimming in a fish bowl - year after year.
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- platty252 (Darren Dalton)
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10% for fixation in histology.[/b]
Dam. I was given 37% and was told i would have to dilute it but they didn't know by how much.
I will get back on the phone tomorrow to check the strength needed to preserve some fish organs to be sent to the lab in Galway.
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- JohnH (John)
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John
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- platty252 (Darren Dalton)
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think its 10%. do you have to cut open the fish to preserve organs?
Yes they wont take a live fish.
I have to send (individually) liver, gills, spleen, heart and kidneys.
The spleen will be tricky since the fish are only about 2-2 1/2".
Daragh i might have to drop in tomorrow to get some 10% off you if thats ok.
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- platty252 (Darren Dalton)
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I think 37% is how it's sold (it's quite volatile at high percentages, I think) have you tried goggling it?
John
The formalin i got came from a lab. for fish.
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- JohnH (John)
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John
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- JohnH (John)
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Sorry to 'keep on', but I think you use 10% solution (with remainder water) of the 37% solution you have been provided...does this make sense?JohnH wrote:
I think 37% is how it's sold (it's quite volatile at high percentages, I think) have you tried goggling it?
John
The formalin i got came from a lab. for fish.
John
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- platty252 (Darren Dalton)
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So that would be 3.7% formalin?
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- JohnH (John)
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John
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- scubadim (scubadim)
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if you use the 37% then 1 part of that solution with 2 parts of water should roughly be 10% formalin solution?!
(more like 12) .if that's what Is used in histology it would be sufficient to keep cells "intact"?How long?
I'm curious to know:huh:
Dimitri
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- JohnH (John)
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a] from my Entomologist friend:
"Yes it should be 9 parts water to 1 part of the formalin (37%)"
b] from Ian Millichip:
"Hi John,
Time is against me to give a full reply unfortunately,
but if Formalin is used then there are quite a few precautions that need to be followed:
-the Formalin has to be buffered (possibly slightly on the acidic side, and may depend upon tissue);
-unbuffered formalin is converted to formic acid and that may decalcify specimens (you'll get a mush)
-Formalin is not safe and workers need to consider the irritant effect
-Care needs to be taken with the storage jars....glass with polypropylene lids (and ideally polyethylene lining). Certain other materials will react with the formalin.
- the specimen to perservative ratio is important and the ideal varies from specimen size to specimen size and type.
The specimen would need to be 'fixed' and 'preserved'.
Now, alcohol (95%) is a good preservative but maybe not a great fixative.
Many people use, and it may even be the recommended method for holding specimens during a field trip.
The Herpetological Society of Ireland recommends to people to preserve their animals in alcohol if they are sending them for analysis, dissection or future preservation treatment.
There is little need to buffer the alcohol (so maybe more convenient to use)
Now, most of the notions of preservation to perfection are driven by the needs of museums.....but I would be sure that for 'single person' usage the alcohol preservation may do.
As I have to dash, then here is a link www.adfg.state.ak.us/pubs/afrb/vol3_n2/shiev3n2.pdf
ian"
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- platty252 (Darren Dalton)
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I got the formalin 10% buffered to neutral from aqua-vet Ireland.
They said they use this strength because when it is higher than 10% the samples become harder.
They also supplied the sample containers.
The samples will only be in the formalin for a coupe of days at most.
They just had to go from Dublin to Galway. All going to plan they should be in Galway now.
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- Sean (Fr. Jack)
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That would be a ecumenical matter!!!
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- Sean (Fr. Jack)
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That would be a ecumenical matter!!!
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- platty252 (Darren Dalton)
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